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polyvinylidene difluoride membranes  (Bio-Rad)


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    Bio-Rad polyvinylidene difluoride membranes
    Polyvinylidene Difluoride Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 2919 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+(pvdf)+membrane/Trans-Blot+Turbo+Mini+PVDF+Transfer+Packs/pmc13018931-112-23-27
    Average 96 stars, based on 2919 article reviews
    polyvinylidene difluoride membranes - by Bioz Stars, 2026-09
    96/100 stars

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    SDS Page:

    Article Title: Liver-specific delivery of MDM2 antisense oligonucleotides counteracts diet-induced metabolic-dysfunction-associated steatotic liver diseases
    Article Snippet: Tissues were homogenized in a radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris HCl, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% NP-40, pH7.4), supplemented with 1 μM DTT and a cocktail of protease inhibitors (catalog #HY-K0010, MedChemExpress, USA) and phosphatase inhibitors (catalog # B15002 , Bimake, USA). .. An equal amount of proteins was separated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (catalog #1620177, Bio-Rad). ..

    Article Title: Assessing the therapeutic effects of DPP4 Inhibitors on TGF-β2-induced Lens Opacity.
    Article Snippet: To quantify protein concentration, a bicinchoninic acid assay (BCA, Thermo Fisher Scientific, 23225) was performed. .. Protein samples were subjected to SDS-PAGE (4%–20% Mini-PROTEAN TGX precast protein gels, Bio-Rad, 4561094), followed by transferred to polyvinylidene difluoride (PVDF) membrane (Bio-Rad, 1704272) using the Trans-Blot TurboTM Transfer System (Bio-Rad). .. After blocked with 5% non-fat dry milk for 1 h, the membranes were incubated overnight at 4 ◦C with primary antibodies against Fibronectin (1:1000, Abcam, ab2413), Vimentin (1:1000, Abcam, 92547), p-Smad2 (1:1000, Cell Signaling Technology, #), Smad2 (1:1000, Cell Signaling Technology, #) and internal control glyceraldehde 3-phosphate dehydrogenase (GAPDH, 1:1000, Cell Signaling Technology, 2118S).

    Article Title: HIV-1 Vpr is targeted for degradation by autophagy.
    Article Snippet: Supernatants were collected, mixed with 2x SDS sample buffer (SigmaAldrich, S3401), and boiled for 10 min on a heat block. .. Proteins were then separated by electrophoresis on 12% SDS-PAGE PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014020 March 2, 2026 29 / 35 polyacrylamide gels and transferred to a polyvinylidene difluoride (PVDF) membrane (BioRad, #1620177) using a TransBlot Turbo Transfer System (Bio-Rad, #1704150EDU). .. Membranes were then incubated for 1 h with blocking buffer (BioRad, #1706404) at room temperature, followed by an overnight incubation at 4 oC with a primary antibody against our protein of interest (antibody sources and dilutions are detailed in Table 2).

    Membrane:

    Article Title: Liver-specific delivery of MDM2 antisense oligonucleotides counteracts diet-induced metabolic-dysfunction-associated steatotic liver diseases
    Article Snippet: Tissues were homogenized in a radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris HCl, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% NP-40, pH7.4), supplemented with 1 μM DTT and a cocktail of protease inhibitors (catalog #HY-K0010, MedChemExpress, USA) and phosphatase inhibitors (catalog # B15002 , Bimake, USA). .. An equal amount of proteins was separated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (catalog #1620177, Bio-Rad). ..

    Article Title: Assessing the therapeutic effects of DPP4 Inhibitors on TGF-β2-induced Lens Opacity.
    Article Snippet: To quantify protein concentration, a bicinchoninic acid assay (BCA, Thermo Fisher Scientific, 23225) was performed. .. Protein samples were subjected to SDS-PAGE (4%–20% Mini-PROTEAN TGX precast protein gels, Bio-Rad, 4561094), followed by transferred to polyvinylidene difluoride (PVDF) membrane (Bio-Rad, 1704272) using the Trans-Blot TurboTM Transfer System (Bio-Rad). .. After blocked with 5% non-fat dry milk for 1 h, the membranes were incubated overnight at 4 ◦C with primary antibodies against Fibronectin (1:1000, Abcam, ab2413), Vimentin (1:1000, Abcam, 92547), p-Smad2 (1:1000, Cell Signaling Technology, #), Smad2 (1:1000, Cell Signaling Technology, #) and internal control glyceraldehde 3-phosphate dehydrogenase (GAPDH, 1:1000, Cell Signaling Technology, 2118S).

    Article Title: Pericardial Fluid–Derived Small Extracellular Vesicles from Patients with Coronary Disease Alter the Lipidome of Human Coronary Artery Endothelium
    Article Snippet: To preserve the phosphorylation status of various proteins, the RIPA buffer contained SIGMAFAST protease inhibitor (MilliporeSigma, Burlington, MA; #S8830-2TAB) and PhosSTOP phosphatase inhibitor (MilliporeSigma; #4906837001) cocktails. .. Protein samples were separated on a gradient 4%-20% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad; #4561094) by electrophoresis and then transferred to the polyvinylidene difluoride (PVDF) membrane (Bio-Rad; #1620177). ..

    Article Title: HIV-1 Vpr is targeted for degradation by autophagy.
    Article Snippet: Supernatants were collected, mixed with 2x SDS sample buffer (SigmaAldrich, S3401), and boiled for 10 min on a heat block. .. Proteins were then separated by electrophoresis on 12% SDS-PAGE PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014020 March 2, 2026 29 / 35 polyacrylamide gels and transferred to a polyvinylidene difluoride (PVDF) membrane (BioRad, #1620177) using a TransBlot Turbo Transfer System (Bio-Rad, #1704150EDU). .. Membranes were then incubated for 1 h with blocking buffer (BioRad, #1706404) at room temperature, followed by an overnight incubation at 4 oC with a primary antibody against our protein of interest (antibody sources and dilutions are detailed in Table 2).

    Article Title: Calcitriol attenuates neuroinflammation induced by ischemia/reperfusion via modulation of the ROS/TXNIP/NLRP3 pathway: Evidence from in silico and in vivo studies.
    Article Snippet: .. The protein samples (20 μg) were subjected to denaturation at 95◦C for 5 min, resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and blotted onto a 0.2-μm polyvinylidene difluoride (PVDF) membrane (Immun-BlotTM, Bio-Rad, USA; Cat. No. 162 017777). .. To block nonspecific binding, membranes were treated with 5% Bowen serum albumin (BSA) (Sigma-Aldrich, Cat. No: A-7888) dissolved in tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, then exposed to primary antibodies for 1 h: • Anti-NLRP3 (1:1000, Cat. No: ab263899, Abcam), • Anti-ASC (1:1000, Cat. No: ab307560, Abcam), • Anti-Caspase-1 (1:1000, Cat. No: 83383S, Cell signaling Technology), • Anti-TXNIP (1:1000, Cat. No: 14715S.

    Article Title: Deep homology and design of proteasome chaperone proteins in Candidozyma auris.
    Article Snippet: Western Blotting Protein levels were normalized to 25 μg and boiled in 1x Laemmli sample buffer for 5 minutes before loading on 4-20% SDS-PAGE gels (Bio-Rad, #4561094). .. Following separation, proteins were transferred to polyvinylidene difluoride (PVDF) membrane (Bio-Rad, #162-0177) for 15 minutes using a semi-dry transfer system. .. Blots were blocked with 5% bovine serum albumin (Sigma-Aldrich, A7030) in phosphate-buffered saline with 0.1% Tween-20 (PBS-T).

    Article Title: Androgen activity in the male embryonic hindbrain drives lethal PFA ependymoma
    Article Snippet: .. The resulting protein was semi-dry transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, 1704272) using the Trans-Blot Turbo Transfer System (Bio-Rad, 1704150), and blocked using 5% skimmed milk in TBST (20 mM Tris, 150 mM NaCl and 0.1% Tween 20, pH 7.5). .. Membranes were incubated with the following primary antibodies overnight: GAPDH (Abcam, ab9484, mouse, 1:600; Abcam, ab9485, rabbit, 1:2,500); lamin B1 (Abcam, ab16048, rabbit, 1:1,000); and AR (Cell Signaling Technology, D6F11, rabbit, 1:1,000).

    Article Title: Androgen activity in the male embryonic hindbrain drives lethal PFA ependymoma
    Article Snippet: .. The resulting protein was semi-dry transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, 1704272) using the Trans-Blot Turbo Transfer System (Bio-Rad, 1704150), and blocked using 5% skimmed milk in TBST (20 mM Tris, 150 mM NaCl and 0.1% Tween 20, pH 7.5). .. Membranes were incubated with the following primary antibodies overnight: GAPDH (Abcam, ab9484, mouse, 1:600; Abcam, ab9485, rabbit, 1:2,500); lamin B1 (Abcam, ab16048, rabbit, 1:1,000); and AR (Cell Signaling Technology, D6F11, rabbit, 1:1,000).

    Electrophoresis:

    Article Title: Pericardial Fluid–Derived Small Extracellular Vesicles from Patients with Coronary Disease Alter the Lipidome of Human Coronary Artery Endothelium
    Article Snippet: To preserve the phosphorylation status of various proteins, the RIPA buffer contained SIGMAFAST protease inhibitor (MilliporeSigma, Burlington, MA; #S8830-2TAB) and PhosSTOP phosphatase inhibitor (MilliporeSigma; #4906837001) cocktails. .. Protein samples were separated on a gradient 4%-20% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad; #4561094) by electrophoresis and then transferred to the polyvinylidene difluoride (PVDF) membrane (Bio-Rad; #1620177). ..

    Article Title: HIV-1 Vpr is targeted for degradation by autophagy.
    Article Snippet: Supernatants were collected, mixed with 2x SDS sample buffer (SigmaAldrich, S3401), and boiled for 10 min on a heat block. .. Proteins were then separated by electrophoresis on 12% SDS-PAGE PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014020 March 2, 2026 29 / 35 polyacrylamide gels and transferred to a polyvinylidene difluoride (PVDF) membrane (BioRad, #1620177) using a TransBlot Turbo Transfer System (Bio-Rad, #1704150EDU). .. Membranes were then incubated for 1 h with blocking buffer (BioRad, #1706404) at room temperature, followed by an overnight incubation at 4 oC with a primary antibody against our protein of interest (antibody sources and dilutions are detailed in Table 2).

    Nucleic Acid Electrophoresis:

    Article Title: Calcitriol attenuates neuroinflammation induced by ischemia/reperfusion via modulation of the ROS/TXNIP/NLRP3 pathway: Evidence from in silico and in vivo studies.
    Article Snippet: .. The protein samples (20 μg) were subjected to denaturation at 95◦C for 5 min, resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and blotted onto a 0.2-μm polyvinylidene difluoride (PVDF) membrane (Immun-BlotTM, Bio-Rad, USA; Cat. No. 162 017777). .. To block nonspecific binding, membranes were treated with 5% Bowen serum albumin (BSA) (Sigma-Aldrich, Cat. No: A-7888) dissolved in tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, then exposed to primary antibodies for 1 h: • Anti-NLRP3 (1:1000, Cat. No: ab263899, Abcam), • Anti-ASC (1:1000, Cat. No: ab307560, Abcam), • Anti-Caspase-1 (1:1000, Cat. No: 83383S, Cell signaling Technology), • Anti-TXNIP (1:1000, Cat. No: 14715S.



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